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Anciently toxicology was known as ‘Science of Poison,’ now a days it is defined as, the study of the effect of chemicals on living organisms and their evaluation as whether these entities are harmful or not or estimating a safe range. For the establishment of safety margins from the adverse effects of chemicals, toxicology depends on few factors like dosage, route of exposure and duration of a certain chemical. The safety level of chemical is decided by the execution of sets of step wise experiments.