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The effects of B vitamins anecdotally are often fairly instantaneous whilst research has found B vitamins (alongside vitamin C and minerals) help participants feel more alert within about 2 weeks. Three months should be long enough to assess whether you are feeling any benefits. But again, it depends on why you are taking vitamin supplements within the first place,.whether you have been diagnosed as deficient during a specific vitamin, or you're just taking them as a precaution A daily B-complex vitamin can help make sure that people that prefer to follow diets that eliminate animal products are becoming enough of those important nutrients.